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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: IL-1β contributes to the secretion of sclerostin by osteocytes and targeting sclerostin promotes spinal fusion at early stages

Fig. 3

Western blotting of ALP, OPN, OCN, and Runx2 protein expression in MC3T3-E1 cells. A Representative western blots. Quantitative analysis of ALP (B), OPN (C), OCN (D), and Runx2 (E) protein expression levels in MC3T3-E1 cells. IL-1β suppressed the protein expression of ALP, OPN, OCN, and Runx2 while inhibiting sclerostin by Ocy454 cells enhanced the protein expression of ALP, OCN, and Runx2 under IL-1β-mediated inflammatory conditions. (F) ALP activity in MC3T3-E1 cells. The experiments in vitro were performed independently 3 times. *p < 0.05 and **p < 0.01. n.s., not significant

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X