Fig. 5

Silencing miR-30b-3p or overexpressing PGK1 reverses the improvement of AS by knocking down HOTTIP or up-regulating miR-30b-3p. A, B The validation of cell transfection. C Proliferation rate of ASFLSs was measured through MTT assay. D. The extracellular calcified nodules of ASFLSs were tested by Alizarin red staining. E, F Expression levels of HOTTIP and PGK1 in AS mice. G Contents of serum TNF-α, IL-6 and IL-β in each group. H Representative images of HE staining. I Representative images of TUNEL staining and the apoptosis rate of synoviocytes in each group. J Expression levels of Bax and Bcl-2 in synovial tissues. *P < 0.05 vs the si-HOTTIP + inhibitor NC group, ^P < 0.05 vs the miR-30b-3p mimic + oe-NC group