Fig. 4

HOTTIP negatively regulates miR-30b-3p and PGK1 is targeted by miR-30b-3p. A Binding sites of HOTTIP and miR-30b-3p. B Binding relation between HOTTIP and miR-30b-3p was confirmed by dual-luciferase reporter gene assay. C Binding relation between HOTTIP and miR-30b-3p was confirmed by RNA pull-down assay. D Binding sites of miR-30b-3p and PGK1. E Target relation between miR-30b-3p and PGK1 was confirmed by dual-luciferase reporter gene assay. N = 3.*P < 0.01 vs the mimic NC group, ^P < 0.05 vs the Bio-NC group