Fig. 3

miR-30b-3p is down-regulated in AS, and down-regulated miR-30b-3p impedes the proliferation and differentiation of ASFLSs. A Expression level of miR-30b-3p in AS patients. B Expression level of miR-30b-3p in ASFLSs. C Proliferation rate of ASFLSs was measured through MTT assay. D The extracellular calcified nodules of ASFLSs were tested by Alizarin red staining. E Expression level of miR-30b-3p in AS mice. F Contents of serum TNF-α, IL-6 and IL-β in each group. G Representative images of HE staining. H Representative images of TUNEL staining and the apoptosis rate of synoviocytes in each group. I Expression levels of Bax and Bcl-2 in synovial tissues. % P < 0.05 vs the control group, # P < 0.05 vs. the mimic NC or agomir NC group