Fig. 4

Expression results of related genes/proteins after adding pathway inhibitors [34]. A–C The mRNA of ATG5, beclin-1 and MMP3 in normal cells, OA cells, OA cells treated with Isorhy and OA cells treated with Isorhy and mTOR inhibitors were detected by qRT-PCR. D–E Western blot was used to detect the expression levels of PI3K, Akt, mTOR, p-PI3K, p-Akt, p-mTOR, ATG5, beclin-1 and MMP3 in normal cells, OA cells treated with Isorhy and mTOR activator (MHY1485). F Immunofluorescence staining results of Beclin-1 and MMP3 after 48 h in four groups of cells, bar: 100 μ m. **P < 0.01, ***P < 0.001 versus OA group, ^#P < 0.05, ^##P < 0.01 versus OA + Isorhy group