Fig. 2

Effects of Isorhy on autophagy and matrix related gene/protein expression A–F Normal cells, OA cells and OA cells treated with different concentrations of Isorhy were discovered by qRT-PCR for ATG5, ATG7, Beclin-1, LC3, MMP3 and MMP13. G Western blotting for ATG5, ATG7, MMP13, Beclin-1, LC3I/LC3II and MMP3 with unequal treatments for 48 h. H Immunofluorescent staining for Beclin-1 and MMP3 with unequal conditionsfor 48 h, bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001