Fig. 5

LXN was a target of miR-127-5p. A The complementary sequences between miR-127-5p and LXN 3′UTR were shown. B Dual-luciferase reporter assay. C RIP assay. D, F The expression of LXN in cartilage tissue and IL-1β-induced chondrocytes was tested by qRT-PCR. E Pearson’s correlation analysis. G, H Western blot was used to examine the expression of LXN in cartilage tissues and IL-1β-induced chondrocytes. I QRT-PCR was used to test assessed the transfection efficiency of miR-127-5p mimic or inhibitor. J, K QRT-PCR and western blot were performed to test LXN mRNA and protein levels. **P < 0.01, ***P < 0.001