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Fig. 8 | Journal of Orthopaedic Surgery and Research

Fig. 8

From: Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis

Fig. 8

MCT1 deletion but not MCT4 significantly reduces circulating tumor cells and distant metastases. A Lung metastases in mice injected with control, MCT1 knockout (KO), MCT4 KO or MCT1/4 KO MNNG/HOS cells and the number of metastatic nodes was counted. Arrows indicated metastases. B Distant metastases that occurred beyond the lung including 3 mice in liver (2 from control group and 1 from MCT4 KO group) and 1 mouse in kidney from control group. Arrows indicated metastases. C. The frequency of circulating tumor cells in blood in mice from A. D Control or MCT1 KO cells were injected directly into the tail vein of mice followed by N-acetyl-cysteine (NAC) treatment or not and the frequency of circulating tumor cells was detected by flowcytometry after weeks. E–F Flow cytometry analysis of MCT1 (E) and MCT4 (F) expression patterns for primary tumor, and metastatic lung, liver and kidney tumor tissues in mice of control group from A. The arrow in E indicated a distinct cell subpopulation with very low expression of MCT1. Data are shown as mean ± standard deviation (SD). Statistical differences are tested by comparison with control group, or as indicated (D). *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant

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