Fig. 7
MCT4 deletion inhibits tumor growth while MCT1 deletion enhances oxidative stress in vivo. A MCT1 and/or MCT4 knockout (KO) MNNG/HOS cells were injected into bone marrow cavity of mouse femur (n = 6 for each group). Primary tumors were harvested and weighted after 4 weeks. B Evaluation of glucose uptake for mice from A (n = 3 for each group) by 18FDG PET/CT examination and tumor site indicated by arrows. C Representative pictures of immunohistochemistry staining of Ki67 and the number of Ki67-positive cells in primary tumor. D Evaluation of lactate levels within primary tumor tissue of mice from A. E–G NAD+/NADH (E), NADP+/NADPH (F) and GSH/GSSG (G) ratios analyzed in primary tumor tissue. H Protein expression levels of MCT1 and MCT4 in peripheral and core region of primary tumor tissue in mice from control group. Data are shown as mean ± standard deviation (SD). Statistical differences are tested by comparison with control group. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant