Fig. 5

Inhibition of MCT1 but not MCT4 impairs metastatic capacity of MNNG/HOS cells by inducing oxidative stress in vitro. A–C NAD+/NADH (A), NADP+/NADPH (B) and GSH/GSSG (C) ratios in MNNG/HOS cells treated with DMSO, 10 µM AZD3965 (AZD), 10 µM VB124 (VB) and AZD + VB (n = 4). D The effects of MCT1 and/or MCT4 inhibitions on ATP production in cells treated with or without N-acetyl-cysteine (NAC) (n = 4). E Cell invasion assessment after MCT1 and/or MCT4 inhibitions and treatment with or without NAC (n = 4). Data are shown as mean ± standard deviation (SD). Statistical differences are obtained by comparison with DMSO group, or with corresponding group without NAC treatment. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant