Fig. 4

The role of MCT1 and MCT4 on tumor cell proliferation, migration and invasion. A Cell proliferation assay in MNNG/HOS and U-2 OS cells treated with 10 µM AZD3965 (AZD) and/or 10 µM VB124 (VB) (n = 5). B Cell proliferation assay in MCT1 and/or MCT4 knockout (KO) cells treated with or without AZD3965 and VB124 (n = 5). C The effect of MCT1 and/or MCT4 inhibitions on cell migration ability (n = 3). D Assessment of invasiveness in MNNG/HOS and U-2 OS cells after MCT1 and/or MCT4 inhibitions (n = 4). E Expression levels of proliferation- and metastasis-related proteins in MNNG/HOS cells treated with DMSO, AZD3965, VB124 and AZD + VB. Data are shown as mean ± standard deviation (SD). Statistical differences are tested by comparison with DMSO group, or as indicated. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant