Fig. 2

Effects of genetic knockout for MCT1 and MCT4 on glycolysis and oxidative respiration. A Protein expression of MCT1 and MCT4 in MNNG/HOS and U-2 OS cells after MCT1 and/or MCT4 knockout. B Extracellular lactate levels in MCT1 and/or MCT4 knockout (KO) cells treated with or without 10 µM AZD3965 (AZD) and 10 µM VB124 (VB) (n = 5). C Oxygen consumption rate (OCR) in MCT1 and/or MCT4 KO cells (n = 5). D Glucose metabolism-related protein expression levels in MCT1 and/or MCT4 KO cells. Data are shown as mean ± standard deviation (SD). Statistical differences are tested by comparison with control group, or as indicated. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant