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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis

Fig. 1

Effects of pharmacological inhibition of MCT1 and MCT4 on glycolysis and oxidative respiration. A Intracellular lactate levels in MNNG/HOS and U-2 OS cells treated with DMSO, 10 µM AZD3965 (AZD), 10 µM VB124 (VB) and AZD + VB for 2 h (n = 5). “24 h” means cells were pretreated with indicated reagents for 24 h and then intracellular lactate level was measured 2 h after replacement of culture medium. B Cells were treated as in A and extracellular lactate level was detected (n = 5). C Cells were treated as in A and basal oxygen consumption rate (OCR) was assessed (n = 5). D Protein expression of MCT1 and MCT4 by western blot analysis after treatment as in A. Data are shown as mean ± standard deviation (SD). In groups with treatment for 2 h, statistical differences are compared to DMSO group. In the groups with treatment for 24 h, statistical differences are compared to corresponding group with 2-h treatment. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant

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