Fig. 5
From: Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
Dual osteogenesis effect of RAPA on BMSCs. A Representative ALP staining images for the three groups at three time points. Scale bar = 200 μm. B Representative images of ARS staining of BMSCs in three groups. Scale bar = 100 μm. C ALP activity. D Quantitative analysis of ARS staining of BMSCs. Gene expression levels of osteogenic markers Alpl, Ibsp, Runx2, Spp1, and Col1a1 on d 7 (E), 14 (F), and 21 (G) after osteogenic differentiation. H Protein levels of ALP, BSP, and RUNX2 on d 7, 14, and 21 after osteogenic differentiation. Quantitative protein expression levels of ALP, BSP, and RUNX2 on d 7 (I), 14 (J), and 21 (K). ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001 (vs. 0 nM)