Fig. 4
From: Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
RAPA gradually induced autophagy in BMSCs during osteogenic differentiation. A Protein levels of BECN1, p62, and LC3-I/LC3-II on d 7, 14, and 21 after osteogenic differentiation. Quantitative BECN1and p62 protein levels and LC3-II/LC3-I ratio on d 7 (B), 14 (C), and 21 (D). E Representative LC3 immunofluorescence images for the three groups at three time points. Scale bar = 25 μm. *p < 0.05, **p < 0.01, ***, p < 0.001 (vs. 0 nM)