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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy

Fig. 3

Cytotoxicity against BMSCs and autophagy levels in BMSCs after treatment with three RAPA concentrations for 24 h. A Cell proliferation after 1, 3, and 5 d of culturing. B LC3 immunofluorescence showing autophagosome formation. Scale bar = 25 μm. C The mRNA expression levels of autophagic markers Becn1, Lc3, and Atg5. D Protein levels of BECN1, p62, and LC3-I/LC3-II in BMSCs. E The LC3-II/LC3-I ratio and quantitative protein levels of BECN1 and p62. *p < 0.05, **p < 0.01, ***p < 0.001 (vs. 0 nM)

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