Fig. 2
From: Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy
Autophagy modulation during BMSC osteogenic differentiation. A ALP and ARS staining in control and induced groups (scale bar = 100 μm). B ALP activity changes during osteogenic differentiation. C–E ALP, BSP, LC3-I/LC3-II, BECN1, and p62 protein levels and their quantitative analysis during osteogenic differentiation. F Relative Lc3, Becn1, and Atg5 gene expression in BMSCs on d 0, 7, 14, and 21 after osteogenic differentiation. G LC3 immunofluorescence showing autophagosome formation on d 0, 7, 14, and 21 after osteogenic differentiation in BMSCs. Scale bar = 25 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (vs. d 0)