Fig. 3

MiR-218-5p facilitates osteogenic differentiation of BMSCs by targeting SOCS3. A SOCS3 protein expression in BMSCs post-transfection of SOCS3. B MiR-218-5p expression in BMSCs post-transfection of miR-218-5p mimics. C Alizarin red staining was used to assess the osteogenic differentiation of BMSCs after miR-218-5p overexpression alone or in combination with SOCS3. D ALP activity in BMSCs after the indicated transfection. E–H The mRNA expression of osteogenesis markers (ALP, OPN, OCN, COL1) in BMSCs transfected with NC mimics, miR-218-5p mimics, miR-218-5p mimics + vector, and miR-218-5p mimics + SOCS3. *p < 0.05; **p < 0.01; ***p < 0.001