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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway

Fig. 6

Upregulation of KIF4A partially reversed the effect of DEPDC1 interference on metastasis and angiogenesis of OS cells. A The migration of U2OS cells transfected with sh-DEPDC1 and Ov-KIF4A was detected by wound healing assay. B The invasion of U2OS cells transfected with si-DEPDC1 and Ov-KIF4A was detected by transwell assay. C Th expression of EMT-related proteins in U2OS cells transfected with sh-DEPDC1 and Ov-KIF4A was detected by western blot. D/E The number of HUVECs tube formation in the supernatant of U2OS cells transfected with sh-DEPDC1 and Ov-KIF4A was analyzed by tube formation assay. F The expression of angiogenesis-related protein in U2OS cells transfected with si-DEPDC1 and Ov-KIF4A was detected by western blot. *P < 0.05 and ***P < 0.001 versus Control group. ##P < 0.01 and ###P < 0.001 versus sh-NC group. ++P < 0.01 and +++P < 0.001 versus sh-DEPDC1 + Ov-NC group

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X