Fig. 2

Immunofluorescence results of S100 (A) and NF200 (B) in sciatic nerve. Pictures were taken using microscope (Olympus CX31, Japan, 40 ×, Scale bar = 50 um). (Right pointing triangle) S100 and PI fluorescent colocalization cell (necrotic SC), (Rightwards arrow) S100 positive cell. C Necrotic SC positive cell rate in the lesion site of sciatic nerve in three groups at 24 h after PNI. D Western blot analysis was performed to study the expression of NF200 in uninjured adult rat sciatic nerve (Sham) and 24 h after a crush lesion (PNI, Nec-1 + PNI). E Semiquantitative analysis (relative density) of the intensity of NF200 to β-actin for each group. The data are mean ± SD (*p < 0.05, comparing the PNI or Nec-1 + PNI group with the Sham group; ^#p < 0.05, comparing the Nec-1 + PNI group with the PNI group)