Fig. 8

Pre-treatment with nesfatin-1 suppressed the excessive autophagy level by blocking of RhoA/ROCK pathway in OA chondrocytes. A Immunofluorescence staining of LC3 and the quantification of mean fluorescence intensity was analyzed by ImageJ. B The expression levels of autophagy correlated proteins including Atg5, Atg7, Beclin1 and LC3 by western blot and analyzed by ImageJ. Data are expressed as means ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with IL-1β group; ^§p < 0.05, ^§§p < 0.01 compared with IL-1β + NES group