Fig. 3

COCH knockdown promotes osteogenic differentiation of HBMSCs. A Transfection efficacy after COCH interference is determined by qRT-PCR. B–F Western blotting (B) and quantitative analysis of COCH (C), RUNX2 (D), Osterix (E), and COL1A1 (F) in HBMSCs treated with si-NC and si-COCH. G, H Staining of calcium deposition by Alizarin Red (G) and quantitative analysis of staining areas (H) in HBMSCs treated with si-NC and si-COCH. Scale bars, 200 μm. I, J Staining of ALP (I) and quantitative analysis of staining areas (J) in HBMSCs treated with si-NC and si-COCH. Scale bars, 200 μm. K, L Immunofluorescence staining of RUNX2 (K) and quantitative analysis of RUNX2-positive nuclei (L) in HBMSCs treated with si-NC and si-COCH. Scale bars, 100 μm. M, N Immunofluorescence staining of Osterix (M) and quantitative analysis of RUNX2-positive nuclei (N) in HBMSCs treated with si-NC and si-COCH. Scale bars, 100 μm. A, C–F, H, J, l, N Values are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA