Fig. 2

COCH is identified as a promising downstream mRNA of miR-1303. A Volcano plots of differentially expressed mRNAs. B Hierarchical clustering heatmap of differentially expressed mRNAs (red: high expression, white: medium expression, and blue: low expression). C Enrichment of top 30 GO terms. D Top 10 enriched KEGG pathways. The node color changes from red to blue in descending order according to the adjusted p values. The size of the node depends on the number of counts. E Chord diagram of the differentially expressed mRNAs according to the results of the GO pathway involved in bone morphogenesis. The box color changes from red to blue in descending order according to the logFC of differentially expressed mRNAs. F Venn diagram of the osteoporosis-related upregulated mRNAs and the predicted mRNAs with the ability to interact with miR-1303. G Verification of the expression pattern of COCH and RARG in the GSE35958. H The expression of COCH and RARG in the osteoporotic subjects and the control subjects is determined by qRT-PCR. G, H Values are shown as mean ± SD. *P < 0.05, **P < 0.01, two-way ANOVA