Fig. 1

GPR84 downregulates in BMMs in bone metastatic model of CRC. A BMMs were isolated from bone marrow in tibias at indicated timepoints post-injection of MC-38 cells. The relative GPR84 levels were determined by qRT-PCR (n = 3). B and C The expression levels of GPR84 in BMMs isolated from bone marrow at indicated timepoints post-injection of MC-38 cells were determined by western blotting assay B and the protein level of GPR84 was normalized to β-actin (C) (n = 3). D and E Representative immunofluorescence images of GPR84 in BMMs isolated from bone marrow in mice at D0 or D7 post-injection of MC-38 cells (D) and quantification of relative expression of GPR84 (E) (n = 3). Bar represents 50 μm. Significant differences are indicated as **p < 0.01 or ***p < 0.001