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Table 1 Lists of primer sequences, amplicon length used for qRT-PCR

From: IRF8 and its related molecules as potential diagnostic biomarkers or therapeutic candidates and immune cell infiltration characteristics in steroid-induced osteonecrosis of the femoral head

Gene symbol

Forward primer (5′–3′)

Reverse primer (5′–3′)

Amplicon length (bp)

Annealing temperature (°C)

GAPDH

GTGAGATCGGTAGGTTGGTG

CCTTGACTTTGAGCTCGTGA

159

60

IRF8

GATCCCTTGGAAACACGCTG

GCCACGCCTAGTTTGCATTT

264

60