Fig. 5

Immunohistochemical analysis of fracture zone at 14, 21 and 28 days after fracture. A Representative images of the fractured femurs stained for BMP-2. B Quantification of the positively stained area in the whole fracture callus for BMP-2. C Representative images of the fractured femurs stained for BMPR-2. D Quantification of the positively stained area in the whole fracture callus for BMPR-2. E Representative images of the fractured femurs stained for RUNX2. F Quantification of the positively stained area in the whole fracture callus for RUNX2. G Representative images of the fractured femurs stained for SMAD4. H Quantification of the positively stained area in the whole fracture callus for SMAD4. (Model group sample data as control, *0.05 > P > 0.01; **0.01 > P > 0.001; ***P < 0.001; n = 3)