Fig. 3
LncRNA CASC9 directly sponging miR-874-3p in OS. A The binding sites of CASC9 on miR-874-3p were predicted by miRDB. B The binding of CASC9 and miR-874-3p was verified by luciferase reporter assay. C The expression levels of miR-874-3p in 30 pairs of OS tumor tissues and normal tissues were detected using qRT-PCR. D The relationship between lncRNA CASC9 expression and miR-874-3p expression was calculated using Pearson’s χ2 test. E, F The expression of miR-874-3p was assessed using qRT-PCR. G The cell proliferation was analyzed using MTT assay. H The cell colony formation activity was examined using cell colony formation assay. I The cell invasion was assessed using transwell assay. **represented P < 0.01