Fig. 1

Total new bone formation 12 weeks post injury. Rats were subjected to a blast injury model with whole-body exposure to blast overpressure, followed by controlled femoral fracture, soft tissue quadriceps crush injury, with 150 min of tourniquet use (Blast Complex HLI 150) or without tourniquet use (Blast Complex Control), and limb amputation through the zone of injury. Micro-computed tomography scans were performed on animals following blast injury with or without tourniquet use at 12 weeks. Total new bone was determined by defining the difference between new bone and naïve bone. Results graphed as mean and 95% CI. Statistical comparison performed using unpaired t-test with Welch’s correction. *p < 0.05; **p < 0.01, ***p < 0.001, ns not significant