Fig. 6

Daphnoretin alleviated OA in mice. The OA mice were intraperitoneally injected with 10 mg/kg of daphnoretin. A–C H&E staining, Safranine O-Fast Green staining and TRAP staining were adopted to observe the morphological differences in the knee sections and the thickness of the subchondral cortical bone plate in mice. D Quantitative analysis of the thickness of the subchondral cortical bone plate in mice. E Quantitative analysis of OARSI scores. F WB was adopted to evaluate the expression of Bax, Bcl2, and cleaved Caspase-3. G–H The profiles of GRP78, CHOP, ATF6, Caspase-12 and NLRP3 inflammasomes were compared by WB. I: qRT-PCR was performed to assess the expression of COX-2, iNOS, TNF-α, and IL-6 in the cartilage tissues. **P < 0.01, ***P < 0.001 (vs. sham group). &P < 0.05, &&P < 0.01, &&&P < 0.001 (vs. OA + veh group). N = 5