Fig. 5

Daphnoretin eased IL-1β-mediated chondrocyte inflammation by inactivating NLRP3 inflammasomes. 10 ng/mL IL-1β was given to mediate chondrocyte apoptosis, and then, 4 μM daphnoretin and/or 1 nM CY-09 were administered on this basis. A qRT-PCR was implemented to clarify the expression of COX-2, iNOS, TNF-α, and IL-6. B The profiles of COX-2 and iNOS were monitored by WB. C The Griess method was applied to test the NO content. D–F The concentrations of TNF-α, IL-6, and PGE2 in the chondrocyte supernatant were examined by ELISA. G WB was performed to verify the NLRP3 inflammasome level. *P < 0.05, **P < 0.01, ***P < 0.001 (vs.IL-1β group). ns P > 0.05 (vs. IL-1β + CY-09 group). N = 3