Fig. 3

BDNF is a directly target of miR-210-3p. A Top ranking list of miR-210-3p target genes obtained from TargetScan software. B The mRNA expression of Bdnf after transfection with miR-210-3p mimics were examined by qRCR. C Relative protein expression of Bdnf were examined after transfection with miR-210-3p mimics or inhibitor. D Binding sites of miR-210-3p and the BDNF 3′UTR, as detected by luciferase reporter assays. BDNF mutation: luciferase reporter plasmid containing mutant BDNF 3′UTR. BDNF -WT: luciferase reporter plasmid containing wild-type BDNF 3′UTR. E Relative luciferase activities of luciferase reporters containing WT or MUT BDNF 3′-UTR in MC3T3-E1 cells transfected with miR-210-3p mimic