Table 2 Primer sequences
From: ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription
Name of primer | Sequences (5′−3′) |
|---|---|
hsa-ZEB1-F | GGCATACACCTACTCAACTACGG |
hsa-ZEB1-R | TGGGCGGTGTAGAATCAGAGTC |
hsa-POLDIP2-F | TACCGAGGTGTCGTCCTGTTTC |
hsa-POLDIP2-R | CCTTTCACCTCCTTGGAGCCAT |
rno-ZEB1-F | CGGCGCAATAACGTTACAAAT |
rno-ZEB1-R | CACTGTCTGGTCTGTTGGCA |
rno-POLDIP2-F | ATCCAGACCACAAGTGCAGG |
rno-POLDIP2-R | GGAACCCTAAGAACTGGGGC |
rno-ALP-F | CCTTAGGGCCACCGCTCG |
rno-ALP-R | GTTCAGTGCGGTTCCAGACA |
rno-OPN-F | CCAGCCAAGGACCAACTACA |
rno-OPN-R | GAACTCGCCTGACTGTCGAT |
rno-RUNX2-F | CCTGAACTCTGCACCAAGTCCT |
rno-RUNX2-R | CATCTGGCTCAGATAGGAGGG |
rno-NFATC-F | GGTGCCTTTTGCGAGCAGTATC |
rno-NFATC-R | CGTATGGACCAGAATGTGACGG |
rno-CTSK-F | AGCAGAACGGAGGCATTGACTC |
rno-CTSK-R | CCCTCTGCATTTAGCTGCCTTTG |
rno-GAPDH-F | CATCACTGCCACCCAGAAGACTG |
rno-GAPDH-R | ATGCCAGTGAGCTTCCCGTTCAG |
rno-β-actin-F | CATTGCTGACAGGATGCAGAAGG |
rno-β-actin-R | TGCTGGAAGGTGGACAGTGAGG |
hsa-β-actin-F | CACCATTGGCAATGAGCGGTTC |
hsa-β-actin-R | AGGTCTTTGCGGATGTCCACGT |
hsa-GAPDH-F | GTCTCCTCTGACTTCAACAGCG |
hsa-GAPDH-R | ACCACCCTGTTGCTGTAGCCAA |
rno-ALDH3B1-F | TTGGCGAGGCCTCAGAATG |
rno-ALDH3B1-R | CTCAGCCTGACTGATGGCAA |
rno-PAF1-F | GAAAGCGGGACCAAGAGGAA |
rno-PAF1-R | GGACCCTGGTCTCTAGCTCA |
rno-ITGAL-F | CCCAGGCTGCAGTTATCACA |
rno-ITGAL-R | AACGCATGCCCTAACCTCAA |
rno-TSR3-F | GGTGAGTTTGGAGCAGTCGG |
rno-TSR3-R | CCAAGCTCCCACATAGCCAA |
rno-NADK-F | TGCAGGGCGATGATTATGCT |
rno-NADK-R | ACTGAGCAAGGCTCTCGAAC |
mmu-ZEB1-F | ATTCAGCTACTGTGAGCCCTGC |
mmu-ZEB1-R | CATTCTGGTCCTCCACAGTGGA |
mmu-POLDIP2-F | AGTGCTGGAGACAGTTGGTGTG |
mmu-POLDIP2-R | CTGGAGTTGCAGAAGCCACATC |
mmu-NFATC-F | GGTGCCTTTTGCGAGCAGTATC |
mmu-NFATC-R | CGTATGGACCAGAATGTGACGG |
mmu-CTSK-F | AGCAGAACGGAGGCATTGACTC |
mmu-CTSK-R | CCCTCTGCATTTAGCTGCCTTTG |
mmu-GAPDH-F | CATCACTGCCACCCAGAAGACTG |
mmu-GAPDH-R | ATGCCAGTGAGCTTCCCGTTCAG |
mmu-POLDIP2 Promoter-F | GCGGAGTTGCGTCTGTATTT |
mmu-POLDIP2 Promoter-R | ACACAGACATCTCCACAGCA |