Fig. 5

Silencing of POLDIP2 attenuates the inhibitory effect of oe-ZEB1 on the differentiation of macrophages RAW264.7 to osteoclasts. RAW264.7 cells were infected with sh-NC, sh-POLDIP2, sh-NC + oe-NC, sh-NC + oe-ZEB1, sh-POLDIP2 + oe-NC or sh-POLDIP2 + oe-ZEB1. A mRNA expression of POLDIP2 and ZEB1 in cells after infection by RT-qPCR. B Detection of protein expression of OPG, NFATC and CTSK in cells after infection by Western blot analysis. C The protein expression of MMP9, c-Fos and Acp5 in cells after infection detected by Western blot analysis. All data are represented as mean ± SD. Each experiment was performed three times independently. *p < 0.05, **p < 0.01. Differences are tested using two-way ANOVA with Tukey’s post hoc test