Fig. 4
From: ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription
POLDIP2 attenuates OP in rats by regulating osteoclast differentiation. A Detection of mRNA expression of POLDIP2 in bone tissues of rats by RT-qPCR. B, C Immunohistochemical detection of OPG (B) and TRAP C expression in bone tissues of rats. D Detection of mRNA expression of NFATC and CTSK in bone tissues of rats by RT-qPCR. E Detection of protein expression of MMP9, c-Fos and Acp5 in bone tissue of rats by Western blot. F Assessment of bone content and structural changes in bone tissue of rats by HE staining where bone trabeculae were labeled with red arrows and bone mass was labeled with green arrows. All data are represented as mean ± SD (n = 6 for rats in each group). Each experiment was performed three times independently. *p < 0.05, **p < 0.01. Differences are tested using two-way ANOVA with Tukey’s post hoc test (D, E) and unpaired t-test (A–C)