Fig. 2
From: ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription
ZEB1 supports osteoblast differentiation and restrains osteoclast differentiation. A Detection of mRNA expression of ZEB1 in bone tissues of rats in response to oe-NC or oe-ZEB1 by RT-qPCR. B Detection of protein expression of ALP, OPN and RUNX2 in bone tissues of rats by Western blot. C Immunohistochemical detection of OPG expression in bone tissues of rats. D Detection of mRNA expression of NFATC and CTSK in bone tissues of rats by RT-qPCR. E Immunohistochemical detection of TRAP expression in bone tissues of rats. F Assessment of bone content and structural changes in rat bone tissue by HE staining where bone trabeculae were labeled with red arrows and bone mass was labeled with green arrows. All data are represented as mean ± SD (n = 6 for rats in each group). Each experiment was performed three times independently. *p < 0.05, **p < 0.01. Differences are tested using two-way ANOVA with Tukey’s post hoc test (B, D) and unpaired t-test (A, C, E)