Fig. 3

RES attenuates IL-1β-induced chondrocyte damage via the SIRT1/FOXO1 pathway. A Western blot shows the expression of target proteins SIRT1, FOXO1, p-FOXO1, aggrecan, SOX9, MMP13, and ADAMTS5, which are associated with the SIRT1/FOXO1 pathway and apoptosis. B The relative expression of SIRT1, FOXO1, p-FOXO1, aggrecan, SOX9, MMP13, and ADAMTS5 was quantified by normalization with GAPDH. C–H The mRNA expression levels of SIRT1, FOXO1, p-FOXO1, aggrecan, SOX9, MMP13, ADAMTS5 in chondrocytes were quantified by quantitative real-time PCR. a–e Corresponds to control, IL-1β, IL-1β + RES, IL-1β + RES + AS, and IL-1β + RES + EX-527, respectively. AS = FOXO1 inhibitor, EX-527 = SIRT1 inhibitor. All data are presented as the mean ± SEM (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001