Fig. 1

The SIRT1/FOXO1 pathway was downregulated in OA cartilage cells from clinical specimens. A, D Western blot analysis shows the expression of target proteins SIRT1, FOXO1, and p-FOXO1. B, E The relative expression of SIRT1, FOXO1, and p-FOXO1 was quantified by normalization with GAPDH. C, F The mRNA expression levels of SIRT1 and FOXO1 in chondrocytes were quantified by quantitative real-time PCR. G, H The concentration of P-Foxo1 protein in cartilage tissues was detected by immunofluorescence, and quantitative immunofluorescence analysis was performed using ImageJ software. All data are presented as the mean ± SEM (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001