Table 2 Specific primers for RT-qPCR
From: Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis
Gene | Primer sequences (5′–3′) |
|---|---|
Circ_0136666 | Forward primer: GGTGCTCACTGTGCTGAAAA Reverse primer: CAGATGTTCATTGGGTCCAT |
MiR-1244 | Forward primer: GCCGAGAAGTAGTTGGTTTG Reverse primer: CTCAACTGGTGTCGTGGA |
CEP55 | Forward primer: GGAGGGCAGACCATTTCAGAG Reverse primer: AGGCTTCGATCCCCACTTAC |
U6 | Forward primer: CTCGCTTCGGCAGCACA Reverse primer: AACGCTTCACGAATTTGCGT |
GAPDH | Forward primer: TATGATGACATCAAGAAGGTGGT Reverse primer: TGTAGCCAAATTCGTTGTCATAC |