Fig. 5

CEP55 is a target of miR-1244 in OS cells. A and B The mRNA targets of miR-1244 were sought using bioinformatics database TargetScan, and we screened six possible mRNA targets for further study. RT-qPCR assay was performed to analyze the expression of six mRNAs (ABCG8, ALDOA, RPL32, PSMB2, CEP55, and PSMC2) in miR-1244-overexpressed OS cells. C and D RT-qPCR and Western blot assay were utilized to analyze the expression of CEP55 in OS tissues and adjacent normal tissues in mRNA and protein levels. E and F The mRNA and protein levels of CEP55 in hFOB 1.19, U2OS and SaOS2 were assessed by RT-qPCR and Western blot assay. G The predicted binding sites between miR-1244 and CEP55 were shown. H and I The interaction between miR-1244 and CEP55 was verified via dual-luciferase reporter assay. J The protein level of CEP55 was determined in miR-1244-overexpressed OS cells by Western blot assay. K The interference efficiency of anti-miR-1244 in OS cells was analyzed via RT-qPCR. L and M OS cells were transfected with si-NC, si-circ_0136666, si-circ_0136666 + anti-NC, or si-circ_0136666 + anti-miR-1244. Western blot assay was utilized to measure the protein level of CEP55 in OS cells. N and O The linear correlation between the expression of CEP55 mRNA and miR-1244 or circ_0136666 was analyzed by Pearson’s correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ^###P < 0.001