Fig. 4

MiR-1244 overexpression restrains the malignant behaviors of OS cells. A–J U2OS and SaOS2 cells were transfected with miR-NC or miR-1244. A miR-1244 expression in transfected cells was assessed via RT-qPCR. B EDU assay was applied to determine the proliferation capacity of OS cells. C The number of colonies in miR-NC group or miR-1244 group was analyzed via colony formation assay to assess cell proliferation ability. D and E The numbers of migrated cells and invaded cells were measured via transwell assays to evaluate cell migration and invasion abilities. F Cell apoptosis rate was determined via flow cytometry. G and H Cell glycolytic metabolism was assessed through measuring the uptake of glucose and the production of lactate using their corresponding kits. I and J The protein levels of HK2 and PKM2 were detected by Western blot assay. ***P < 0.001