Skip to main content
Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis

Fig. 3

MiR-1244 is a target of circ_0136666. A and B The subcellular localization of circ_0136666 was analyzed using the commercial kit. C and D The miRNA targets of circ_0136666 were predicted using bioinformatics software circinteractome, and six candidate miRNAs were screened. RT-qPCR was conducted to measure the expression of six miRNAs (miR-1299, miR-198, miR-579, miR-1244, miR-370, and miR-758) in OS cells transfected with si-NC or si-circ_0136666#1. E The level of miR-1244 was determined in 41 pairs of OS tissues and adjacent normal tissues by RT-qPCR. F RT-qPCR was conducted to measure the expression of miR-1244 in hFOB 1.19 and two OS cell lines (U2OS and SaOS2). G The putative target sequence between circ_0136666 and miR-1244 was shown. H and I The target relationship between circ_0136666 and miR-1244 was tested by dual-luciferase reporter assay. Circ_0136666 WT or circ_0136666 MUT was co-transfected into OS cells with miR-NC or miR-1244. After transfection for 24 h, relative luciferase activity was determined. J Biotinylated RNA-pull down assay was carried out to analyze the target relationship between circ_0136666 and miR-1244 using biotinylated miR-1244 or miR-NC. K The expression of miR-1244 in circ_0136666-silenced OS cells was analyzed using RT-qPCR. L The linear correlation between the levels of circ_0136666 and miR-1244 was assessed by Pearson’s correlation analysis. **P < 0.01, ***P < 0.001

Back to article page