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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Circ_0051079 functions as an oncogenic regulator in osteosarcoma by leading to MAFB expression upregulation by competitively interacting with miR-1286

Fig. 6

MiR-1286 functioned as a tumor repressor in OS cells by reducing MAFB expression. A, B RT-qPCR and western blot were used for MAFB quantification in pcDNA or MAFB transfection group. C–J SaoS-2 and U2OS cells were transfected with miR-1286, miR-1286 + MAFB or matched controls. C–E CCK-8 assay (C, D) and Edu assay (E) were used to determine cell proliferation. F Flow cytometry was used for examination of apoptosis rate. G Western blot was applied to detect levels of apoptotic proteins. H, I Transwell assay was applied for analysis of cell migration (H) and invasion (I). J Western blot was exploited for protein detection of EMT-associated markers. *P < 0.05

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