Fig. 4

Inhibition of miR-1286 counterbalanced anti-tumor response of si-circ_0051079 in OS cell progression. A Inhibition efficacy of anti-miR-1286 was evaluated via RT-qPCR assay. B–I SaoS-2 and U2OS cells were performed with si-NC, si-circ_0051079, si-circ_0051079 + anti-miR-NC, si-circ_0051079 + anti-miR-1286 transfection. B–D Cell proliferation was analyzed through CCK-8 assay (B, C) and Edu assay (D). E Apoptosis rate was examined using flow cytometry. F Apoptotic markers were determined by western blot. G, H Migrated (G) and invaded (H) cells were detected via transwell assay. I EMT-associated proteins were measured using western blot. *P < 0.05