Fig. 2

OS cell proliferation, migration, and invasion were inhibited but apoptosis was promoted after the silence of circ_0051079. Si-NC and si-circ_0051079 were transfected into SaoS-2 and U2OS cells, respectively. A Level detection of circ_0051079 was carried out by RT-qPCR. B–D Measurement of cell proliferation was taken via CCK-8 assay (B–C) and Edu assay (D). E Examination of apoptosis rate was conducted using flow cytometry. F Protein detection of apoptotic markers was implemented via western blot. G–H Assessment of migration (G) and invasion (H) was performed by transwell assay. I Determination of EMT-related proteins was conducted through western blot. *P < 0.05