Fig. 6

Role of the NEAT1-miR-338-3p-GLS axis in FLSs-RA. A FLSs-RA was transfected with control, NEAT1 alone, or in combination with miR-338-3p, B protein expression of GLS was examined by qRT-PCR and Western blot, respectively. C Glutamine uptake assay and D glutaminase activity were measured from the above-transfected cells. E FLSs-RA were transfected with control, NEAT1 alone or in combination with miR-338-3p, cells were treated with H₂O₂ at the indicated concentrations for 48 h and cell viability was determined by MTT assay and F Annexin V apoptosis assay. Significant differences between the two groups were identified using the Mann–Whitney U test. Values are means (± SD, n = 3). *p < 0.05, **p < 0.01