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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Inhibition of lncRNA NEAT1 induces dysfunction of fibroblast-like synoviocytes in rheumatoid arthritis via miRNA-338-3p-mediated regulation of glutamine metabolism

Fig. 5

Restoration of GLS restored miR-338-3p-mediated glutamine metabolism and cellular dysfunction in FLSs-RA. A FLSs-RA were transfected with control, miR-338-3p alone, or GLS plus, and GLS expression was examined by Western blot. B Glutamine uptake and C glutaminase activity were detected from the above-transfected cells. D FLSs-RA cells transfected with control, miR-338-3p alone, or plus GLS were treated with H2O2 at the indicated concentrations for 8 h, and cell viability was determined by MTT assay. Significant differences between the two groups were identified using the Mann–Whitney U test. Values are means (± SD, n = 3). *p < 0.05, **p < 0.01, ***p < 0.001

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