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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Inhibition of lncRNA NEAT1 induces dysfunction of fibroblast-like synoviocytes in rheumatoid arthritis via miRNA-338-3p-mediated regulation of glutamine metabolism

Fig. 4

miR-338-3p directly targets the 3' UTR of the GLS of FLSs. A miR-338-3p binding sites on the 3' UTR of GLSs were predicted from StarBase. B The expression of GLS mRNA in normal FLSs and FLSs-RA was detected by qRT-PCR. C FLSs-RA cells were transfected with control siRNA or GLS siRNA for 48 h, and cells were treated with the indicated concentration of H2O2. Cell viability was examined by MTT assay. D FLSs-RA cells were transfected with control miRNA or miR-338-3p, and protein expression of GLS was detected by Western blot. E WT- or Mut-3'UTR of GLS was transfected into normal FLSs and F FLSs-RA with control miRNA or miR-338-3p, and a luciferase reporter assay was performed. Luciferase activity was examined; significant differences between the two groups were identified using the Mann–Whitney U test. Values are means (± SD, n = 3). *p < 0.05, **p < 0.01

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