Fig. 1

correlation of NEAT1 and miR-338-3p with cellular dysfunction and glutamine metabolism in FLSs-RA. A Expression of NEAT1 in FLSs from healthy donors and RA patients. B FLSs-RA cells without or with NEAT1 silencing by NEAT1 siRNA transfection were treated with the indicated concentrations of H₂O₂ and cell viability was determined by MTT assay, C Annexin V assay. D Glutamine uptake and E glutaminase activity were examined in FLSs-RA cells without and with NEAT1 silencing. F Predicted NEAT1-miR-338-3p interaction from StarBase. G Expression of miR-338-3p in FLS of healthy donors and RA patients. H FLSs-RA cells that did or did not overexpress miR-338-3p were treated with the indicated concentrations of H₂O₂, and cell viability was determined by MTT assay and I Annexin V assay. J Glutamine uptake and K glutaminase activity were examined in FLSs-RA cells without or with overexpression of miR-338-3p. Significant differences between the two groups were identified using the Mann–Whitney U test. Values are means (± SD, n = 3). *p < 0.05, **p < 0.01