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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Circular RNA circ_0008365 regulates SOX9 by targeting miR-338-3p to inhibit IL-1β-induced chondrocyte apoptosis and extracellular matrix degradation

Fig. 3

MiR-338-3p was a target of circ_0008365. A The binding sequence between circ_0008365 and miR-338-3p was predicted by starbase online database; B The expression level of miR-338-3p was detected by qRT-PCR in CHON-001 cells transfected with miR-NC or miR-338-3p; C, D The luciferase activity in 293 T and CHON-001 cells co-transfected with miR-NC/miR-338-3p and WT-circ_0008365/MUT-circ_0008365 was detected by dual-luciferase reporter assay; E The enrichment of circ_0008365 in CHON-001 cells incubated with Bio-NC or Bio-miR-338-3p was detected by RNA pull-down assay; F The enrichments of circ_0008365 and miR-338-3p were measured by RIP assay; G The miR-338-3p expression in OA cartilage tissues (n = 25) and normal tissues (n = 20) was measured by qRT-PCR; H Pearson correlation analysis was carried out to reveal the relationship between miR-338-3p and circ_0008365 expression in OA tissues; I The expression of circ_0008365 in CHON-001 cells treated with IL-1β, IL-1β + pcDNA or IL-1β + circ_0008365 and control cells was detected by qRT-PCR assay. ***P < 0.001

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