Fig. 2

Circ_0008365 overexpression inhibited IL-1β-induced CHON-001 cell damage. A The expression of circ_0008365 was detected by qRT-PCR in CHON-001 cells transfected with pcDNA or circ_0008356; B–F CHON-001 cells were divided into 4 groups, including con, IL-1β, IL-1β + pcDNA, and IL-1β + circ_0008356 groups; (B) CCK-8 assay was used to detect cell viability; C EDU assay was used to measure cell proliferation; D Flow cytometry analysis was performed to evaluate the apoptosis of cells; E, F Western blot assay was employed to detect the protein levels of proliferation-related PCNA, apoptosis-related pro-caspase-3 and cleaved caspase-3 and ECM-related protein (MMP13, ADAMTS5, COL2A1, and Aggrecan). **P < 0.01, ***P < 0.001